Composite
Part:BBa_K3425057
Designed by: Tereza Hubackova Group: iGEM20_UofUppsala (2020-10-25)
IPTG inducible expression construct of Juniper GFP
BBa K3425057 is an IPTG inducible expression construct of Juniper GFP constructed from the LacI regulated promoter, BBa_K1847014, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 496
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 496
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 496
Illegal BamHI site found at 705 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 496
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 496
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |