Composite

Part:BBa_K3425057

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)


IPTG inducible expression construct of Juniper GFP

BBa K3425057 is an IPTG inducible expression construct of Juniper GFP constructed from the LacI regulated promoter, BBa_K1847014, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BamHI site found at 705
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None